Our research lines have as main purpose the study of C. albicans-host interaction, with the final goal of contributing with new strategies to fight against invasive candidiasis. We are interested in the study of C. albicans proteome along the interaction with macrophages and under oxidative stress conditions. We are focused on quantitative proteomics, protein complexes (Interactome) and posttranslational modifications of proteins (PTMs). Changes in phosphorylation and acetylation will be studied in depth, since they are some of the most relevant PTMs during cell signaling. These studies will be performed using Data Independent Analysis (DIA), which is an up to date proteomic technique that enables a high throughput analysis.
The proposed projects are:
1. Monitoring the changes in the phosphoproteome and acetylome during C. albicans-macrophage interaction and in the presence of H2O2 as apoptosis inducer. Mass spectrometry analysis, data mining and functional validation of the results. These results will shed light to C. albicans biology and to the signaling networks involved in its death, which could be applied for the design of new therapies
2. Global proteomic study in C. albicans after treatment with metformin, an oral antidiabetic drug that was recently found to have synergic antifungal activity with common antifungal drugs. Mass spectrometry analysis, data mining and functional validation of the results.
3. Study of C. albicans interactome, using native electrophoresis, mass spectrometry and machine learning will allow an integrated description of Candida cellular processes and of the important proteins involved in this response, including those with unknown function. Six hours of interaction with macrophages will be the selected condition for this study